TABLE 2.

Predicted sizes of restriction fragments used for RFLP analysis of eae

Intimin typeExpected fragment size(s) (bp) with the following restriction enzyme:
AluIRsaICfoI
Int-α1736, 113725, 84,a 40a437, 412
Int-α2375, 334, 133, 7a590, 135, 84, 40a437, 412
Int-β475, 374528, 246, 75a618, 231
Int-γ834 (uncut)432, 402834 (uncut)
Int-ε384, 270, 190, 32a774, 102345, 260, 195, 76a
Int-ζ605, 203, 38a345, 279, 135, 87a603, 225, 18a
Int-η384, 270, 190, 32a774, 102421, 260, 195
Int-θ527, 165, 110, 21,a 14a405, 354, 78a837 (uncut)
Int-ι602, 241525, 318843 (uncut)
Int-κ342, 214, 162, 131231, 201, 159, 120, 84,a 54a624, 225
Int-λ232, 214, 204, 151, 45a441, 318, 87a846 (uncut)
Int-μ824, 19a525, 318843 (uncut)
Int-ν844, 32a774, 102876 (uncut)
Int-ξ247, 214, 161, 148, 79a321, 318, 126, 84a313, 311, 225
  • a The fragment was too small to visualize under the electrophoresis conditions used.