TABLE 2.

Initial and resolved sensitivity and specificity of the BTUB FRET assay

Data typePCRSensitivity (%)SpecificityPPVNPV
InitialaTETR100 (20/20)100 (233/233)100 (20/20)100 (233/233)
BTUB FRET75.0 (15/20)98.3 (229/233)78.9 (15/19)97.8 (229/234)
RevolvedbTETR81 (17/21)98.7 (232/235)85.0 (17/20)98.3 (232/236)
BTUB FRET90.4 (19/21)100 (232/232)100 (19/19)99.1 (232/234)
  • a Sensitivity and specificity of BTUB FRET compared to TETR PCR.

  • b Sensitivity and specificity where infection status is determined by two positive PCR results, BTUB FRET and TVK3/4 PCR (14).