TABLE 1.

Primers used for amplification and sequencing of the ankA gene

No. of ticksFirst PCRNested PCRAdditional primers used for sequencinga
Primers usedReference or sourcePrimers usedReference or source
15 of 42U7 + 1R132U8 + 1R732FvL ankU3
U5 + 1R432FvL ankU1
U3 + 1R232FvL ankU2
15 of 421F + 4R1321F + 1R32FvL ank1
2F1 + 2R132FvL ank2
3F + 3R32FvL ank3
4F1 + 4R132
15 of 424F2 + D2324F3 + D132
8 of 42U8 + 1Rmod32; this studyU8 + 1R7mod32; this study
U5mod + 1R4modThis study
U3mod + 1R2modThis studyU3 Seq1
U3 Seq2
U3 Seq3
U3 Seq4
8 of 421F + 4R1mod32; this study1Fmod2 + 1RmodThis study
2F1 mod + 2R1modThis study
8 of 421Fmod2 + D1This study; 323Fmod + D132; this study3Fmod Seq1
3Fmod Seq2
3Fmod Seq3
3Fmod Seq4
3Fmod Seq5
3Fmod Seq6
8 of 424F2 + D2324F3 + D132
1 of 42U7 + 1R132U8 + 1R732FvL ankU3
U5 + 1R432FvL ankU1
U3 + 1R232FvL ankU2
1 of 421F + 4R1mod32; this study1F + 1R32
2F1neu + 4RneuThis study2F1neu Seq1
2F1neu Seq2
2F1neu Seq3
2F1neu Seq4
4F1neu Seq1
4F1neu Seq2
4F1neu Seq3
4F1neu Seq4
4F1neu Seq5
1 of 424F2neu + D132; this study
  • a Primers were designed for this study and are listed in Table 2.