TABLE 2.

Nucleotide sequences of primers used for amplification and sequencing of the ankA gene

PrimerNucleotide sequence
Amplification
    U5modGCA AGC ACG TGA GGC AGC G
    U3modGCG TAT GGA TCA CGA GAA AA
    1Fmod2GTA TTC TGC AGT CTG CTC TTA G
    1RmodCTT AGT GCT TCA GCG GTC AG
    1R2modGCG TAG GAA ACT TAG AAC TA
    1R4modCAT ACT GCA CTG CAC TCA TCC
    1R7modATA TCT GCT CCA TAC CAC TG
    2F1modCAC TGA AGT GGT TGC TGT AA
    2R1modGAA TAT AAA GCC TCA TGT AAC G
    2F1neuCAA GAG ACA ACT CTG GCT TTT
    3FmodGCT GCA AGC CGC TCC ATT CT
    4F2neuCAG AAG GAT TAC AGG GAG CA
    4R1modCTT TGA GGA GCT TCT GGT TG
    4RneuGGA TCC TGC TTT GCC TTT TC
Sequencing
    FvL ankU1CAC TCC GCT TCA TAT TGC TA
    FvL ankU2ACT CCT GAG TCT GTT GTA AA
    FvL ankU3TAA CAC ATC CTC TTT CAA CC
    FvL ank1GAA GAA ATT ACA ACT CCT GAA
    FvL ank2GCA GCT GCT AAT GGT GAC GG
    FvL ank3TAT CAT CCT TGG GTA GTG G
    U3 Seq1GCT CAG GTT CCC CAG AAG TA
    U3 Seq2AAT TTT GGC TAT CAT TAG AAG AT
    U3 Seq3GAA CAC CCT CAA TCA ACA TC
    U3 Seq4CAT CTC CAG TGA CAG GTG TA
    2F1neu Seq1GGA TCT ATG GAT GAA CAA GG
    2F1neu Seq2ATT AAC AAG TGC CAG GTG TAA
    2F1neu Seq3CAG TAA TGA TTC TCC GCT TG
    2F1neu Seq4GTA TCA CCA GAA TGT TTC CT
    3Fmod Seq1CCA TTT GCT CTT TGC TGA GAA
    3Fmod Seq2CTG GGG CAA CAG CAT CAG T
    3Fmod Seq3CAC TGG AGA ATA AAG AAG TAG G
    3Fmod Seq4ATG CAA CAG TGA AAA AGG GTC
    3Fmod Seq5CTT TTT GAG AAG TAT CAC TC
    3Fmod Seq6GCT GAA GGT CTA GGA ACG CC
    4F1neu Seq1GTC AGC ATA GAT AGA TTC AGG
    4F1neu Seq2AGA AGA AAT AGA TTC CCA AGT A
    4F1neu Seq3GCC TAT CTA CGA GGA CAT TA
    4F1neu Seq4TAG AAG AGC GTG TCC AAG TA
    4F1neu Seq5TCT TTC TTC TTA ACC ACA GAT A