TABLE 1.

PCR primers used to serotype L. monocytogenes strains

Primer setForward primer sequenceReverse primer sequenceProduct size (bp)Anneal temp (°C)Specificity
D1aCGATATTTTATCTACTTTGTCATTGCTCCAAAGCAGGGCAT21459Division I or III
D2aGCGGAGAAAGCTATCGCATTGTTCAAACATAGGGCTA14059Division II
FlaAbTTACTAGATCAAACTGCTCCcAAGAAAAGCCCCTCGTCC53854Serotypes 1/2a and 3a
GLTbAAAGTGAGTTCTTACGAGATTTAATTAGGAAATCGACCTTCT48345Serotypes 1/2b and 3b
  • a PCR products run on a 2% agarose gel.

  • b PCR products run on a 1.2% agarose gel.

  • c The underlined nucleotide is a mismatch that was introduced to increase primer specificity.