TABLE 1.

Reproducibility of the BTUB FRET PCR assay

TechnicianStandard (known concn)Mean calculated concn (SD)a,bMean Ct (SD)a,c
11 (84)88.1 (21.4)30.2 (0.40)
2 (42)38.8 (5.65)31.5 (0.23)
3 (21)19.4 (3.81)32.6 (0.35)
4 (10.5)12.2 (1.50)33.3 (0.21)
5 (5.25)6.50 (3.30)34.5 (0.78)
6 (1.06)0.84 (0.03)37.6 (0.05)
21 (84)91.1 (21.6)31.0 (0.45)
2 (42)45.6 (10.5)32.4 (0.44)
3 (21)22.3 (3.80)33.5 (0.33)
4 (10.5)10.6 (2.47)34.7 (0.40)
5 (5.25)4.48 (0.95)36.4 (0.39)
6 (1.06)1.62 (1.10)38.4 (1.30)
Pooled means1 (84)89.6 (21.5)30.6 (0.43)
2 (42)40.7 (8.10)31.9 (0.33)
3 (21)20.8 (3.80)33.1 (0.34)
4 (10.5)11.4 (1.90)34.1 (0.30)
5 (5.25)5.40 (2.10)35.5 (0.60)
6 (1.06)1.23 (0.60)38.0 (0.70)
  • a All values are means based on five replicates analyzed for each technician and 10 replicates analyzed for the pooled means.

  • b T. vaginalis organisms per PCR, generated by the LightCycler utilizing Ct and the known concentration of the standards.

  • c Ct was generated based on the cycle number where the LightCycler software interpreted a logarithmic increase in fluorescence of the given sample.