TABLE 1.

Oligonucleotide primer pairs used for development of SQ-PCR assay for R. rickettsii

Target genePrimer designationNucleotide sequence 5′→3′Amplified fragment size (nt)Tm (°C)Reference
gltA RpCS877F GGG GAC CTG CTC ACG GCG G 3816815
RpCS1258R ATT GCA AAA AGT ACA GTG AAC A 58
rompA RR190.70F ATG GCG AAT ATT TCT CCA AAA A 6325817
RR190.701R GTT CCG TTA ATG GCA GCA TCT 62
RR190.70F ATG GCG AAT ATT TCT CCA AAA A 5325815
RR190.602R AGT GCA GCA TTC GCT CCC CCT 68
RR190.70F ATG GCG AAT ATT TCT CCA AAA A 7758This study
RR147.147R CTA CTC AGC ATT ATC GCT GCG 64
RR190.70F ATG GCG AAT ATT TCT CCA AAA A 9758This study
RR190.167R ACA CCT GTA GCA ACA CCG AGT 64
RR190.547F CCT GCC GAT AAT TAT ACA GGT TTA 15466This study
RR190.701R GTT CCG TTA ATG GCA GCA T 62
RR190.588F GGA GCG AAT GCT GCA CTA AT 11360This study
RR190.701R GTT CCG TTA ATG GCA GCA TCT 62