TABLE 1.

PCR primers and conditions used in this study

Primer pairSequenceTargetPCR conditionsaLength of PCR product (bp)Reference
DenaturingAnnealingExtension
KS75′-CCC GGA TCC ATG AAA AAA ACA TTA TTA ATA GC-3′ stxB 1 94°C, 30 s52°C, 60 s72°C, 40 s282 26
KS85′-CCC GAA TTC AGC TAT TCT GAG TCA ACG-3′ stxB 1c
GK35′-ATG AAG AAG ATG TTT ATG-3′ stxB 2 94°C, 30 s52°C, 60 s72°C, 40 s260 13
GK45′-TCA GTC ATT ATT AAA CTG-3′ stxB 2c
VT2-cm5′-AAG AAG ATA TTT GTA GCG G-3′ stxB 2d 94°C, 30 s55°C, 60 s72°C, 60 s256 24
VT2-f5′-TAA ACT GCA CTT CAG CAA AT-3′
SK15′-CCC GAA TTC GGC ACA AGC ATA AGC-3′ eae 94°C, 30 s52°C, 60 s72°C, 60 s863 26
SK25′-CCC GGA TCC GTC TCG CCA GTA TTC G-3′
Stx1c-1b5′-TTT TCA CAT GTT ACC TTT CCT-3′ stxA 1c 94°C, 30 s51°C, 60 s72°C, 60 s498This study
Stx1c-25′-CAT AGA AGG AAA CTC ATT AGG-3′
Oligo 15′-TCG CAT GAG ATC TGA CC-3′ stx 1, stx1c94°C, 30 sc55°C, 60 s72°C, 90 s1,469 22
Oligo 25′-AAC TGA CTG AAT TGA GAT G-3′Whole
  • a All PCRs included 30 cycles followed by a final extension step of 5 min at 72°C.

  • b Primers Stx1c-1 and Stx1c-2 were derived from the positions 511 to 531 and 988 to 1008, respectively, of the stx1c sequence from strain 3115/97 determined in this study (accession no. AJ312232 ).

  • c PCR conditions were established in this study.