TABLE 2.

Oligonucleotides used in this study

Primer or probe nameTarget geneOligonucleotide sequence (5′ → 3′)aAmplicon size (bp)
Primers
    1Slt224 stx 1 ATG TCA GAG GGA TAG ATC CA185
    1Slt385 stx 1 TAT AGC TAC TGT CAC CAG ACA AT
    2Slt537 stx 2 AGT TCT GCG TTT TGT CAC TGT C160
    2Slt678b stx 2 CGG AAG CAC ATT GCT GAT T
Probes
    1SltB1FAM stx 1 GCG AGG CGC TTT GCT GAT TTT TCA CAT GTT ACC CCT CGC
    2SltB1TET stx 2 GCG AGG CAC TGT CTG AAA CTG CTC CTG TCC TCG C
  • a The underlined sequences of each probe constitute the stem of each molecular beacon. FAM, 6-carboxyfluorescein; TET, tetrachloro-6-carboxyfluorescein.