TABLE 1.

Times and temperatures used for the PCR configuration with SYBR Green I and the LC

ParameterTemp (°C)Time (s)Slope (°C/s)Acquisition mode
Denaturation95820None
Amplification (40 cycles)951020None
561020None
72820Single
Melting951020None
673020None
95100.1Continue
Cooling406020None