TABLE 1.

Primers used for PCRs in this study

PrimerSequenceFragment length (kb)a
NK95′-CCACCAGCTGCAGCCATA-3′1.2
NK115′-TGATGCTAATAATGAATCTAAAATGGTAAC-3′
NK1045′-GTGTAGCAATGAAAGTCCAAGTTTACGC-3′0.2
NK1055′-CACTTAGCTCTTTGATTGCTGCACCT-3′
A1C5′-GGAGGTTTTTATGCTTTAATATCTAAAGA-3′3.1
A2N5′-CCCTCTGTTATTGTAGGTAGTACATTTA-3′
A2C5′-TAAATGTACTACCTACAATAACAGAGGG-3′2
A3N5′-CTTGTATATAAATCAGGTGCTATCAATA-3′
A3C5′-TATTGATAGCACCTGATTTATATACAAG-3′3.1
A4N5′-TTATCAAACATATATTTTAGCCATATATC-3′
16S5′-GTGCGGCTGGATCACCTCCT-3′
23S5′-CCCTGCACCCTTAATAACTTGACC-3′
  • a For the toxin gene of C. difficile strain VPI 10463.