TABLE 3.

WAVE gradient parameters

StepaTime (min)b% Buffer
ABD
Loading0.051490
Step 10.546540
Step 21.042580
Step 31.540600
Step 42.538620
Step 53.537630
Step 64.537630
Step 75.534660
Step 86.532680
Start clean6.600100
Stop clean7.100100
Start equilibrate7.251490
Stop equilibrate8.151490
  • a Sequence of events for loading the column, gradient changes, and cleaning the column.

  • b Time of each gradient change.