TABLE 1.

B. pertussis genes analyzed for polymorphism

GeneAlleleNo. of strains analyzed% Frequency (n)GenBank accession no.
ptxS1 a 196 M13223
ptxS1A 79 (154) AJ245366
ptxS1B 17 (33) AJ245367
ptxS1D 4 (7) AJ245368
ptxS1E 1 (2) AJ006151
ptxS2 a 21 M13223
ptxS3 a 196
ptxS3A 78 (153) M13223
ptxS3B 22 (43)AJ420987
ptxS4 a 22 M13223
ptxS5 a 22 M13223
prn b 196
prn1 34 (67) AJ011091
prn2 41 (80) AJ011092
prn3 22 (43) AJ011093
prn4 0.5 (1) AJ011015
prn5 1.5 (3) AJ011016
prn6 1 (2) AJ132095
fim2 a 19
fim2-1 84 (16) Y00527
fim2-2 16 (3)AJ420988
fim3 a 12 X51543
brkA a 5 U12276
fhaB c 22
fhaB-1 86 (19) M60351
J04531
X52156
fhaB-2 14 (3) AJ420989
ompP a 18 X58488
ompQ a 6
ompQ1 17 (1) U16266
ompQ2 83 (5) AJ420990
tcfA d 196
tcfA1 1 (2) U16754
tcfA2 80 (157) AJ009785
tcfA3 18 (36) AJ420991
tcfA5 0.5 (1) AJ420992
vag8 a 6
vag8-1 17 (1) U90124
vag8-2 83 (5) AJ420993
bipA a 6
bipA1 83 (5)P. Cotter
bipA2 17 (1)This study
  • a Genes sequenced completely.

  • b prn region 1 was sequenced for all strains, and region 2 was sequenced for 20% of the strains.

  • c The fhaB gene from 13 strains was sequenced, between bases 1 and 6612. This is the region that codes for the secreted, processed, molecule incorporated in ACVs. For the remaining strains, the region comprising bases 2250 to 2750 and containing the polymorphic locus was sequenced.

  • d The tcfA gene from five strains was sequenced completely. Subsequently, the region between bases 395 and 945 was sequenced for all strains. The region between bases 1 and 394 was sequenced for one of five Dutch strains and for all strains from outside The Netherlands.