TABLE 2.

RT-PCR conditions

Reaction component (concn unit)Concn for:
MBGV/ EBOVLASVCCHFVRVFVDENVYFV
Additional MgSO4 (mM)a0.752.53.252.53.252.5
Sense primer (μM)10.30.2510.150.25
Antisense primer (μM)10.20.5b10.3b0.25
Probe (μM)0.20.20.2
  • a The reaction mix contains an MgSO4 basis concentration of 1.2 mM which is not included.

  • b Consists of a 1:1 molar ratio of two primers.