TABLE 1.

B. anthracis isolates tested for presence of pagA (plasmid pX01) and capB (plasmid pX02)

IsolatepX01/pX02LightCycler assay result
pagAcapB
AmespX01+/pX02+++
BC3132pX01/pX02++
delta-AmespX01/pX02++
GT3pX01+/pX02+++
GT10pX01+/pX02+++
GT15pX01+/pX02+++
GT20pX01+/pX02+++
GT23pX01+/pX02+++
GT25pX01+/pX02++a
GT28pX01+/pX02+++
GT29pX01+/pX02+++
GT30pX01+/pX02+++
GT34pX01+/pX02+++
GT35pX01+/pX02+++
GT38pX01+/pX02+++
GT41pX01+/pX02+++
GT45pX01+/pX02+++
GT51pX01+/pX02+++
GT55pX01+/pX02+++
GT57pX01+/pX02+++
GT62pX01+/pX02+++
GT68pX01+/pX02+++
GT69pX01+/pX02+++
GT77pX01+/pX02+++
GT80pX01+/pX02+++
GT85pX01+/pX02+++
GT87pX01+/pX02+++
Mayo 1pX01+/pX02+++
SD96-1-355pX01+/pX02+++
SternepX01+/pX02+
VollumpX01+/pX02+++
ZimbabwepX01+/pX02+++
  • a Initial genotyping of this strain at the AFIP showed the presence of the capA gene. Subsequent testing in the present study did not reveal the presence of the capB gene, suggesting that the plasmid pX02 was lost by the organism. This was confirmed, because PCR assays for all encapsulation genes (capA, capB, and capC) were negative.