TABLE 3.

Comparison of diagnostic positivity by cell culture versus RT-PCR assays for the direct detection of EV71 from clinical specimensa

TechniqueFraction of true-positive specimens (%)
Cell culture isolation7/35 (20)
RT-PCR with pan-enterovirus primers (5′ UTR)b25/35 (71)
Seminested PCR with EV71 type-specific primers (VP1)17/32 (53)
RT-PCR with EV71 type-specific primers (VP1)c1/21 (4.8)
RT-PCR EV71 type-specific primers (VP1)d1/21 (4.8)
  • a A total of 51 clinical specimens were studied. Seminested PCR with EV71-specific primers was performed for only 48 samples, since three non-EV71 samples were excluded. Single-round RT-PCR with EV71 primers was carried out on only 21 selected specimens. True-positive specimens refer to those from which viable or nonviable EV71 was detected by at least one of the methods.

  • b Universal pan-enterovirus primers targeting the 5′ UTR (10, 15, 16).

  • c EV71 type-specific primers targeting the VP1 region described by Singh et al. (12).

  • d EV71 type-specific primers targeting the VP1 region described by Brown et al. (3).