TABLE 2.

Cell culture isolates tested by single-round RT-PCR with EV71 type-specific primers

Cell culture isolate(s)No. of samples testedNo. of positive samples with primers:
VP1F2-EV71R2a159S-162Ab92S-93Ab
EV71/7423/MS/87 (reference strain)1111
EV71 outbreak strains2222
EV71 endemic strains1915190
Coxsackievirus A163002
Echovirus 302001
  • a EV71 type-specific primers designed by Singh et al. (12).

  • b EV71 type-specific primers designed by Brown et al. (3).