TABLE 1.

PCR primers used in identification of resistance genes

GeneOligonucleotide sequencesaProduct size (bp)Reference or source
bla PSE1 F, TTT GGT TCC GCG CTA TCT G; R, TAC TCC GAG CAC CAA ATC CG150 9
bla TEM F, GCA CGA GTG GGT TAC ATC GA; R, GGT CCT CCG ATC GTT GTC AG310 9
aphA1-lab F, AAA CGT CTT GCT CGA GGC; R, CAA ACC GTT ATT CAT TCG TGA500 14
aadA F, GTG GAT GGC GGC CTG AAG CC; R, AAT GCC CAG TCG GCA GCG528 24
strA F, CTT GGT GAT AAC GGC AAT TC; R, CCA ATC GCA GAT AGA AGG C548 AF273682
strB F, ATC GTC AAG GGA TTG AAA CC; R, GGA TCG TAG AAC ATA TTG GC509 AF024602
sulA F, CAC TGC CAC AAG CCG TAA; R, GTC CGC CTC AGC AAT ATC360 AF071555
tetA F, GCT ACA TCC TGC TTG CCT TC; R, CAT AGA TCG CCG TGA AGA GG210 X61367
tetB F, TTG GTT AGG GGC AAG TTT TG; R, GTA ATG GGC CAA TAA CAC CG659 19
tetG F, CAG CTT TCG GAT TCT TAC GG; R, GAT TGG TGA GGC TCG TTA GC844 19
grm F, AAG CGC ACG AAG CGC GGG CTG; R, AAG GCG GGC CTC AAG GAG GTC414 14
aadB F, GAG CGA AAT CTG CCG CTC TTG; R, CTG TTA CAA CGG ACT GGC CGC310 14
aac(6)-I F, TGA GCA TGA CCT TGC GAT; R, GAA CAG CAA CTC AAC CAG337 AF282595
int F, GGC ATC CAA GCA GCA AG; R, AAG CAG ACT TGA CCT GAVariable 19
  • a F, forward; R, reverse.