TABLE 1.

Percentages of isolates determined to be fluconazole susceptible, susceptible-dose dependent, and resistant among the 793 Candida spp. tested as determined by the NCCLS microdilution broth method and six commercial systemsa

Method and species (no. of isolates)% of isolates
SusceptibleSusceptible-dose dependentResistant
Microdilution broth
    All isolates83.618.078.32
    C. albicans (478)90.13.26.7
    Non-C. albicans (315)74.015.710.3
Sensititre YeastOne
    All isolates81.6912.945.46
    C. albicans (478)93.13.63.4
    Non-C. albicans (315)64.127.28.7
Etest
    All isolates89.393.916.69
    C. albicans (478)93.11.05.9
    Non-C. albicans (315)83.88.38.0
Disk diffusion
    All isolates82.3510.347.31
    C. albicans (478)96.72.11.3
    Non-C. albicans (315)60.622.916.5
Fungitest
    All isolates86.299.014.70
    C. albicans (478)92.65.32.1
    Non-C. albicans (315)76.614.78.7
Candifast
    All isolates14.5417.0768.39
    C. albicans (478)6.117.476.5
    Non-C. albicans (315)27.416.656.1
Integral System Yeasts
    All isolates35.6957.766.56
    C. albicans (478)40.658.21.3
    Non-C. albicans (315)28.357.114.6
  • a Results of the reference method, Etest, Sensititre YeastOne, and disk diffusion were classified as susceptible, susceptible-dose dependent, or resistant according to the MIC breakpoints recommended for fluconazole in the M27-A protocol (7). For the remaining systems tested, the isolates were classified as susceptible, intermediate, or resistant in accordance with the individual manufacturer's instructions. For the purpose of analysis, the isolates classified with the conceptually similar term intermediate by Fungitest, Candifast, and Integral System Yeasts were included in the susceptible-dose dependent category.