TABLE 4.

Discrepancies between the reference method and commercial systems in the classification of 793 Candida isolates based on NCCLS-defined breakpoints for fluconazole susceptibilitya

Method and organisms tested (no. of isolates)No. of isolates with discordant results
Minor errorsMajor errorsVery major errors
S vs. SDDSDD vs. SSDD vs. RR vs. SDD
Sensititre YeastOne
    C. albicans (478)111027722
    Non-C. albicans (315)49175967
Etest
    C. albicans (478)313112227
    Non-C. albicans (315)113376514
Disk diffusion
    C. albicans (478)91220231
    Non-C. albicans (315)52181432012
Fungitest
    C. albicans (478)231120833
    Non-C. albicans (315)23326121313
Candifast
    C. albicans (478)7901143250
    Non-C. albicans (315)437364110
Integral System Yeasts
    C. albicans (478)2486122410
    Non-C. albicans (315)13491117267
  • a Discrepancies were considered major errors if an isolate classified as susceptible (S) by the reference method was classified as resistant (R) by the commercial method, very major errors if an isolate classified as resistant by the reference method was classified as susceptible by the commercial method, and minor when susceptible versus susceptible-dose dependent (SDD), resistant versus susceptible-dose dependent, susceptible-dose dependent versus susceptible, or susceptible-dose dependent versus resistant results emerged.