TABLE 1.

Oligonucleotide primers and probes used in PCRs and hybridization assays

Oligonucleotide primer or probeSequenceTarget organismTarget geneNucleotide position (range)Reference or source
Primers
    16S8FEGGAATTCAGAGTTGGATCMTGGYTCAGEubacteria16S rRNA gene8-27L. M. Schouls
    B-GA1B5′biotin-CGGGATCCCGAGTTTGCCGGGACTTCTTCTAnaplasma/Ehrlichia genus16S rRNA gene476-456L. M. Schouls
    44k-startATGATGTCTATGGCTATAGTCATGA. phagocytophila group44-kDa genea49-72This study
    44k-endATCTCCCACAACACGATGA. phagocytophila group44-kDa genea1180-1197This study
    16SEhrSeqGGAATTCAGAGTTTGATCMTGGYTCAGAACEubacteria16S rRNA gene8-30This study
    GA1BSeq        CGGGATCCCGAGTTTGCCGGGACTTCTTCTGTAAnaplasma/Ehrlichia genus16S rRNA gene476-459This study
Probes
    A-EhrA115′ amino-TTATCGCTATTAGATGAGCCAnaplasma/Ehrlichia genus16S rRNA gene203-222L. M. Schouls
    A-HGE5′ amino-GCTATAAAGAATAGTTAGTGGA. phagocytophila prototype16S rRNA gene87-107L. M. Schouls
    A-Phago5′ amino-TTGCTATAAAGAATAATTAGTGGA. phagocytophila variant 116S rRNA gene85-107L. M. Schouls
    A-Phagovar25′ amino-GAACGGATTATTCTTTGTAGCA. phagocytophila variant 216S rRNA gene87-106This study
    A-D-Phago5′ amino-TTGCTATGAAGAATAATTAGTGA. phagocytophila variant 316S rRNA gene87-106L. M. Schouls
    A-D-HGE5′ amino-GCTATGAAGAATAGTTAGTGA. phagocytophila variant 416S rRNA gene87-106L. M. Schouls
    A-Eschot5′ amino-GCTGTAGTTTACTATGGGTAEhrlichia sp., Schotti variant16S rRNA gene76-95L. M. Schouls
    A-Ecan5′ amino-TCTGGCTATAGGAAATTGTTAE. canis16S rRNA gene85-105L. M. Schouls
    A-Echaf5′ amino-ACCTTTTGGTTATAAATAATTGTTAE. chaffeensis16S rRNA gene83-107L. M. Schouls
    A-EmurisC5′ amino-GCTATAGGTTCGCTATTAGE. muris16S rRNA gene85-103A. N. Alekseev
    A-TmpBEhr5′ amino-GAAAACTCGAAGAACAAAGAAT. pallidumbtmpB502-522A. N. Alekseev
  • a Based on the sequence carrying the 44-kDa gene (AF037599) published by Ijdo et al. (10).

  • b That is, Treponema pallidum.