TABLE 2.

Analytical accuracy of the Encephalitozoon LC PCR assay for the detection of E. intestinalis-spiked fecesa

Assay and storage conditions% Positive results at the following concn of spores/ml
102103104105106107
LC PCR
    Fresh5088100100100100
    Refrigerated (4°C for 72 h)50100100100100100
    Frozen (−70°C for 7 days)256375100100100
    ECOFIX (room temperature for 14 days)0075100100100
Smear with trichrome blue stain (no PCR)000075100
  • a Fecal samples spiked with microsporidian spores at the indicated levels were analyzed by using the indicated assays. MDLs are indicated by bold type. Eight spiked fecal samples were analyzed at each concentration.