Stability of cryopreserved cells (RCFM) for the detection of viruses

Cell typeaNo. of cells/shell vial positive forb:
Fresh19, 215, 745, 6114, 22
RCFM 3m18, 227, 1136, 2738, 20
RCFM 4m16, 184, 852, 4950, 101
  • a RCFM 3m, after 3 months at −80°C; RCFM 4m, after 4 months at −80°C.

  • b Number of infected blue cells positive for HSV and number of infected immunofluorescent cells positive for FluA and FluB. Results from duplicate shell vials for each cell type are shown.