TABLE 2.

Detection of C. pneumoniae in aliquots of porcine aortic tissue samples in four laboratories by three different DNA extraction protocols

SampleAmt of chlamydiae/PCR mixtureaPCR result by the following DNA extraction protocol for the indicated laboratoryc
No. of IFUNo. of EBsbBM kitQ kitK protocol
ABCDABCDABCD
S1103,500+++++++++++
S200
S31350+++++++++++
S400
S50.135+++++++++++
S600+
S70.013.5+++++++++++
S800
S90.0051.75++++++++++
S1000
  • a Amount of chlamydiae (numbers of IFU corresponding to numbers of elementary bodies) in a 10-μl amplified DNA extract/50 μl of first-round PCR mixture.

  • b EBs, elementary bodies.

  • c +, reported as C. pneumoniae positive by nested PCR; −, reported as C. pneumoniae negative by nested PCR.