B. henselae isolates studieda

Isolate(s)Source16S typegltA typeAP typeERIC typeIRS type
Feline isolates (n = 31)
    HC35*, HC55BloodIIHHE3a1
    HC41, HC48†, HC61, HC63†, HC77BloodIIMME22
    RMC6†, RMC9†BloodIIMME52
    RMC7, Berlin-2BloodIIMME52
Human isolates (n = 28)
    ATCC 49793/NZRm3492 (s4)BAIHHE4H1
    ATCC 49882 (Houston-1)†, Berlin-1BAIHHE4H2
    BH3(s4), BH5s2*, R987, JR1, JR2, JR3, JR5, JR6, JR7, JR8, JR9, JR12, JR13, JR14, JR15, JR17, JR18, JR19, JR20, JR22, JR23CSDIHHE4H2
    URLLY-8 (Marseille)†BAIIMME4M2
  • a Symbols: *, Isolate embeds into solid agar and displays ratchet motility at this passage number; †, 16S type was determined by direct sequencing. s2, second passage after initial isolation; (s4), third to fifth passage after initial isolation. If not specified, RMC-prefix isolates (this work) were studied at passage numbers 2 to 3; HC-prefix isolates were a gift of Tom Gottlieb (8) and were studied at passage numbers 4 to 6; NU-prefix isolates (15) were studied at passage numbers 3 to 5; R- and JR-prefix strains (this work and Fournier et al. [12a]) were all studied at passage numbers 4 to 7. Other isolates were isolated in our laboratory or obtained from the following: Mardjan Arvand, Hygiene-Institute, Heidelberg, Germany (2) (Berlin-1 and -2); R. Bunter, Goulburn Valley Base Hospital, Goulburn, NSW, Australia (BH2); the New Zealand quality control strain NZRm3492 is thought to be a lab-passaged derivative of the widely used strain ATCC 49793, and they are inseparable by all typing methods; Jock Harkness, Royal North Shore Hospital, Sydney, Australia [BH3(s4)]; T.H.E. Pathology, Fairfield Heights, NSW, Sydney, Australia [BH5(S2)]; and D. Raoult, Unite des Rickettsies, Marseille, France (9) (URLLY-8 [Marseille]). Strains with the ATCC prefix were obtained from the American Type Culture Collection, Manassas, Va. 16S-23S regions were sequenced in all feline strains, in all Sydney and imported human strains, and in JR2.