TABLE 4.

Clinical evaluation of three different DNA extraction protocols for detection of C. pneumoniae DNA in endarterectomy specimens after exchange of DNA extracts among four laboratories (laboratories A to D)a

Panel 3 sampleNo. of C. pneumoniae-positive PCR results by method and laboratory for:
DNA extraction performed at laboratory ADNA extraction performed at laboratory BDNA extraction performed at laboratory CDNA extraction performed at laboratory D
BMQKBMQKBMQKBMQK
ABCDABCDABCDABCDABCDABCDABCDABCDABCDABCDABCDABCD
Atheroma (n = 15)0011002000000010002000001112400002220001000010000
Negative control (n = 5)001100130000001000000000022300011101001000000000
  • a Each sample was tested by PCR 48 times, with a total of 960 analyses performed: 720 and 240 analyses with identical aliquots from 15 endarterectomy samples and 5 negative controls, respectively.