Predicted sizes of restriction fragments and enzymes used for restriction fragment length polymorphism analysis of amplified products of multiplex PCR

GenePCR amplicon size (in bp)EnzymeExpected size of restriction fragments (bp)
hipO323BsrDI109, 214
C. coli glyA126AluI11, 36, 79
C. lari glyA251ApoI79, 172
C. upsaliensis glyA204DdeI31, 173
C. fetus subsp. fetus-sapB2435BclI130, 305
C. jejuni 23S rRNA650HhaI212, 438