TABLE 6

Performance of the real-time PCR in predicting antimicrobial resistance to four antibiotic classes directly from Neisseria gonorrhoeae-positive extragenital specimens with paired N. gonorrhoeae isolatesa

ReactionAssociated antibioticNo. of isolates with the following result:Sensitivity (95% CI)bSpecificity (95% CI)b
TPFPFNTNTotal
penA Asp345del ± penA Gly545SerESCs015c03954NA0.72 (0.58, 0.84)
23S rRNA C2611T and/or 23S rRNA A2059GAZM0105354NA0.98 (0.90, 1.00)
gyrA Ser91PheCIP231030541.00 (0.85, 1.00)0.97 (0.83, 1.00)
16S rRNA C1192TSPC0205254NA0.96 (0.87, 1.00)
  • a CI, confidence interval; TP, true positive; FP, false positive; FN, false negative; TN, true negative; ESCs, extended-spectrum cephalosporins; AZM, azithromycin; CIP, ciprofloxacin; SPC, spectinomycin; NA, not applicable.

  • b Sensitivity and specificity were calculated on the basis of the susceptibility phenotype (MICs) of the paired culture isolates.

  • c Two isolates were correctly predicted to harbor a mosaic penA allele on the basis of the molecular characterization but were susceptible to ESCs.