TABLE 1

Prototheca sp. strains used in this study

Strain group and Species/genotypegStrainaSourceGenBank accession no(s). (clone no.)fReference
SSUITSLSUcytb
Type strains
    P. zopfii genotype 1SAG 2063bSwine manure, GermanyMF163506 (1), MF163507 (2)MF163488 (1), MF163489 (2), MF163490 (3), MF163491 (4)KY654530MF1634643
    P. zopfii genotype 2SAG 2021bBovine mastitis, GermanyMF163508 (1), MF163509 (2), MF163510 (3)MF163492 (1), MF163493 (2), MF163494 (3)MH374907MF163469
    P. blaschkeaeSAG 2064bHuman onychomycosis, GermanyMF163511 (1), MF163512 (2), MF163513 (3)MF163498 (1), MF163499 (2), MF163500 (3)KY654532MF163449
    P. cutisATCC PRA-338cHuman dermatitis, JapanMF163514 (1), MF163515 (2)MF163476 (1), MF163477 (2), MF163478 (3), MF163479 (4)AB470469MF1634534
    P. ulmeaATCC 50112cSap from wounded elm (Ulmus amercicana), USAMF163516 (1), MF163517 (2), MF163518 (3)MF163495 (1), MF163496 (2), MF163497 (3)AB097094MF163456
    P. stagnoraATCC 16528cDigested sludge, USAMF163519 (1), MF163520 (2), MF163521 (3)MF163485 (1), MF163486 (2), MF163487 (3)AB097091MF163455
    P. wickerhamiiATCC 16529cHousehold plumbing, USAMF163504 (1), MF163505 (2)MF163474 (1), MF163475 (2), MF163483 (3), MF163484 (4)AB183198MF163459
    P. miyajiiIFM 53848dHuman dermatitis, JapanMF163522 (1), MF163523 (2), MF163524 (3), MF163525 (4)MF163480 (1), MF163481 (2), MF163482 (3)KP898385MF1634545
    P. tumulicolaJCM 31123eSoil from a stone chamber, JapanMG906573 (1), MH130092 (2), MH130093 (3)MG910494 (1), MH130090 (2), MH130091 (3)LC090551MG9117196
Confirmed strains
    P. zopfii genotype 118/IV*Rectal swab, cow, PolandNDNDMH374897MF163465
48/IV*Cow's water bowl, PolandMH374898MF163466
70/IV*Cow manure, PolandMH374899MF163467
    P. zopfii genotype 2P535**Bovine mastitis, GermanyMH374900MF163471
P232**Canine protothecosisMH374901MF163470
POL1*Bovine mastitis, PolandMH374902MF163472
    P. blaschkeaeBEL-2***Bovine mastitis, BelgiumMH374903MF163451
BEL-7***Bovine mastitis, BelgiumMH374904MF163452
49/IV*Cow's water bowl, PolandMH374905MF163450
    P. wickerhamiiPW-A*Skin lesion (Cyprinus carpio), PolandMF163526 (1)MF163501 (1), MF163502 (2), MF163503 (3)KX828712ND13
PWPL1*Human neuroinfection, PolandNDNDJX256253MF16346135
201/09****Human disseminated algaemia, MalaysiaMH374906MF16346075
  • a Sources of confirmed strains are indicated as follows: *, in-house collection; **, Uwe Roesler, Institute of Animal Hygiene and Environmental Health, Centre for Infectious Medicine, Freie Universität Berlin, Germany; ***, Paul-Émile Lagneau, Regional Association for Health and Animal Identification, Ciney, Belgium; ****, Ratna Mohd Tap, Bacteriology Unit, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.

  • b Culture Collection of Algae at the University of Göttingen (SAG), Göttingen, Germany.

  • c American Type Culture Collection (ATCC), Manassas, VA, USA.

  • d Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan.

  • e Japan Collection of Microorganisms (JCM) RIKEN Hirosawa, Wako-shi, Saitama, Japan.

  • f Numbers in parentheses indicate different clones derived from a single Prototheca strain. A number of the LSU sequences have been previously published, as indicated (†). ND, not determined.

  • g See the text for the distinctions between the two groups of strains.