TABLE 2

Molecular differentiation of Prototheca species/genotypes by PCR-RFLP analysis with RsaI and TaiI and with MboI of the cytb gene fragment

Enzyme(s) and species/genotypeLength of fragment(s) (bp)a
Determined in silicoExpected upon gel electrophoresis
RsaI/TaiI
    P. zopfii genotype 125, 47, 253, 31950, 250, 320
    P. zopfii genotype 225, 47, 161, 41150, 160, 410
    P. blaschkeae22, 25, 25, 61, 68, 93, 128, 22260, 70, 90, 130, 220
    P. cutis25, 25, 129, 183, 282130, 180, 280
    P. ulmea140, 247, 257140, 250
    P. stagnora25, 140, 479140, 480
    P. wickerhamii25, 25, 89, 94, 41190, 400
    P. miyajii25, 619620
    P. tumulicola15, 629620
MboI
    P. miyajii151, 221, 272150, 220, 270
    P. tumulicola644640
  • a The sizes were rounded to the nearest 10. Fragments of ca. 20 bp in length were excluded since they are hardly visible even in high-percentage (4%) agarose gels.