TABLE 1

Characteristics of 8 compared tests for the detection of IgM or IgG anti-Borrelia antibodies

MethodaType of assaybKit, manufacturercAntigensd
Whole-cell antigens
    WEE-IgM/IgGELISAAnti-Borrelia ELISA (IgM)/Anti-Borrelia plus VlsE ELISA (IgG), EuroimmunAntigen extracts of Borrelia burgdorferi sensu stricto, Borrelia afzelii, and Borrelia garinii; IgG enriched with recombinant VlsE of Borrelia burgdorferi
    WSA-IgM/IgGEIA species-specificEIA Borrelia afzelii IgM/EIA Borrelia afzelii VlsE IgG, TestLineSonicated whole-cell antigen of Borrelia afzelii with high content of p83, p41 (flagellin), p39, OspA, OspC, p18 and p14; IgG enriched with VlsE
    WSB-IgM/IgGEIA species-specificEIA Borrelia burgdorferi sensu stricto IgM/EIA Borrelia burgdorferi sensu stricto VlsE IgG, TestLineSonicated whole-cell antigen of Borrelia burgdorferi sensu stricto with high content of p83, p41 (flagellin), p39, OspA, OspC, p18 and p14; IgG enriched with VlsE
    WSG-IgM/IgGEIA species-specificEIA Borrelia garinii IgM/EIA Borrelia garinii VlsE IgG, TestLineSonicated whole-cell antigen of Borrelia garini with high content of p83, p41 (flagellin), p39, OspA, OspC, p18 and p14; IgG enriched with VlsE
Recombinant antigens
    RCL-IgM/IgGCLIA chemiluminescence immunoassayLiaison Borrelia IgM II/IgG, DiaSorinRecombinant VisE; IgM enriched with OspC
    RET-IgM/IgGEIAEIA Borrelia recombinant IgM/IgG, TestLineCombination of selected parts of specific antigens, as follows: OspC, internal flagelin-p41 a p39 of Borrelia burgdorferi sensu lato species; IgG enriched with p83 and p17 (IgG)
    REV-IgM/IgGELISAELISA-Viditest anti-Borrelia recombinant IgM/IgG (CSF), VidiaMixture of specific recombinant antigens
    RBT-IgM/IgGBlot-LineBlot-Line Borrelia/HGA IgM, TestLineRecombinant antigens, as follows: p41 Ba, p39 Ba, OspC Ba, OspC Bg, OspC Bs, and p44
Blot-Line Borrelia/HGA IgG, TestLineRecombinant antigens, as follows: VlsE Ba, VlsE Bg, VlsE Bs, p83 Ba, p41 Ba, p39 Ba, OspB Bs, OspA Ba, OspA Bg, OspA Bs, OspC Bg, p17 Bg, p44, and TpN17
  • a WEE, ELISA based on a whole-cell antigen (Euroimmun); WSA, ELISA based on a whole-cell antigen, specific to B. afzelii; WSB, ELISA based on a whole-cell antigen, specific to B. burgdorferi; WSG, ELISA based on a whole-cell antigen, specific to B. garinii; RCL, CLIA based on a recombinant antigen (DiaSorin); RET, EIA based on a recombinant antigen (TestLine); REV, ELISA based on a recombinant (Vidia); RBT, Blot-Line test based on a recombinant antigen (TestLine).

  • b ELISA, enzyme-linked immunosorbent assay; EIA, enzyme immunoassay; and CLIA, chemiluminescent immunoassay.

  • c HGA, human granulocytic anaplasmosis.

  • d The data on the antigens used were taken from the manufacturer's instructions.