TABLE 1

Primers/probes and oligonucleotide sequences

NameSequenceTma (°C)Oligonucleotide length (bp)Amplicon length (bp)
Forward primerTGACGAGCTGCGAGC6215
Reverse primerATGTGCTCGACGCG6014
Probe50FAM-GCACGTAGCGCGACGC-BHQ681650
Probe100FAM-TACGTGCACGTAGCCGAGACTC-BHQ692256
Probe150FAM-AGGCACTTCTATCGCTGGCTCAC-BHQ702357
Probe200FAM-TAGATACTGGGTCAACAGCGAGGC-BHQ692458
Probe500FAM-CGAGTAAGGCTAGCATCGCTGC-BHQ692256
Probe1500FAM-GCAGCTGTGAGCATACTACGGAC-BHQ672357
50-bp fragmentTGACGAGCTGCGAGCATCGCACGTAGCGCGACGCTAATGTGCTCGACGCGb
  • a Tm, melting temperature.

  • b Shading indicates the locations of the primers and probe in the sequence.