TABLE 1

Identification of infective endocarditis pathogens by different methodological approaches

Sample no.Heart valve sample typeResult with FilmArray BCID multiplex PCR approachResults with routine diagnostic approachesMatches routine approach? (no. of results matching)
Gram stainingCulture16S rRNA gene sequencing
1AorticEnterococcus sp.Gram-positive cocciE. faecalisE. faecalis✓ (3)
2AorticEnterococcus sp.Gram-positive cocciE. faecalisNegative✓ (2)
3AorticEnterococcus sp.Gram-positive cocciE. faecalisNegative✓ (2)
4MitralEnterococcus sp.NegativeE. faecalisNegative✓ (2)
5AorticEnterococcus sp.Gram-positive cocciE. faecalisE. faecalis✓ (3)
6MitralStaphylococcus aureusGram-positive cocciNegativeS. aureus✓ (2)
7MitralStaphylococcus aureusGram-positive cocciS. aureusS. aureus✓ (3)
8AorticStaphylococcus aureusNegativeNegativeS. aureus✓ (1)
9MitralStaphylococcus aureusGram-positive cocciS. aureusS. aureus✓ (3)
10AorticStaphylococcus aureusNegativeS. aureusNegative✓ (3)
11MitralStaphylococcus aureusGram-positive cocciNegativeS. aureus✓ (2)
12AorticStaphylococcus aureusGram-positive cocciS. aureusS. aureus✓ (3)
13AorticStaphylococcus aureusGram-positive cocciS. aureusS. aureus✓ (3)
14AorticStaphylococcus sp.Gram-positive cocciNegativeS. epidermidis✓ (2)
15AorticStaphylococcus sp.Gram-positive cocciS. epidermidisS. epidermidis✓ (3)
16MitralStaphylococcus sp.Gram-positive cocciNegativeS. epidermidis✓ (2)
17AorticStreptococcus agalactiaeGram-positive cocciS. agalactiaeS. agalactiae✓ (3)
18MitralStreptococcus sp.NegativeNegativeS. sanguinis✓ (1)
19AorticStreptococcus sp.Gram-positive cocciS. gallolyticusS. gallolyticus✓ (3)
20MitralNegativeNegativeNegativeNegative✓ (3)
21MitralNegativeNegativeNegativeNegative✓ (3)
22MitralNegativeNegativeNegativeNegative✓ (3)
23AorticNegativeNegativeNegativeNegative✓ (3)
24AorticNegativeNegativeNegativeNegative✓ (3)
25MitralNegativeNegativeNegativeNegative✓ (3)
26MitralNegativeNegativeNegativeNegative✓ (3)
27MitralNegativeNegativeNegativeNegative✓ (3)
28AorticNegativeNegativeNegativeNegative✓ (3)
29AorticNegativeNegativeNegativeNegative✓ (3)
30MitralNegativeNegativeNegativeNegative✓ (3)
31AorticNegativeNegativeNegativeNegative✓ (3)
32MitralNegativeNegativeNegativeNegative✓ (3)