TABLE 2

Performance estimates of studiesa on blood- and urine-based cfDNA detection of M. tuberculosis by nucleic acid amplification techniques in which the methodological steps are a priori considered suitable for cfDNA isolation and detectionb

Publication first authorYrSample typeTB presentationHIV positive (%)Smear positive (%)Method of TB confirmation% (no./total no. of samples) of indicating:
SensitivitySpecificity
Ushio2016PlasmaPulmonary0100Culture65 (21/33)93 (18/19)
29 (10/33)100 (19/19)
Click2018PlasmaPulmonary64100Culture and/or Xpert45 (18/40)67 (2/3)
Cannas2008UrinePulmonary595Sputum smear or culture79 (34/43)100 (23/23)
Fortun2014UrinePulmonary12NRCulture18 (5/28)NR
Extrapulmonary29NR70 (57/82)NR
Labugger2017UrinePulmonary060Culture64 (7/11)100 (8/8)
Patel2017UrinePulmonary3833Culture43 (75/175)89 (210/237)
  • a See references 23, 24, 25, 32, 33, and 34.

  • b NR, not reported; Xpert, Xpert MTB/RIF assay.