TABLE 3

Molecular characterization of 61 isolates of Candida auris from Korean hospitals

YearSource/sample no.Hospital (no. of isolates)FRaErg11pbMLST results for 4 alleles (ITS-RPB1-RPB2-D1/D2)ePFGE typec
AASGenBank accession no. or similar isolateAllele profileGenBank accession no. or similar isolateST clusterEKREAG-N
1996Blood/B1A (1)RNoneMK294623a-a-a-aMK294578-MK294608-MK294593-MK2945632K1N1a
2006Ear/E4A (1)SNoneMK308826a-a-a-aMK308751-MK308776-MK308801-MK3087262K1N1a
Ear/E5A (1)RNoneMK308828a-a-a-aMK308753-MK308778-MK308803-MK3087282K1N1b
Ear/E6A (1)RNoneMK308829a-a-a-aMK308754-MK308779-MK308804-MK3087292K1N1c
Ear/E7A (1)RNoneMK308830a-a-a-aMK308755-MK308780-MK308805-MK3087302K1N1d
Ear/E8A (1)RNoneMK308831a-a-a-aMK308756-MK308781-MK308806-MK3087312K1N1c
EarA (1)SNoneMK308827a-a-a-aMK308752-MK308777-MK308802-MK3087272K1N1e
EarA (1)RNoneMK308832a-a-a-aMK308757-MK308782-MK308807-MK3087322K1N1f
EarA (1)RNoneMK308833a-a-a-aMK308758-MK308783-MK308808-MK3087332K1N1g
2007EarB (1)SNoneMK308834a-a-a-aMK308759-MK308784-MK308809-MK3087342K1N1h
EarB (1)SNoneMK308835a-a-a-aMK308760-MK308785-MK308810-MK3087352K1N1c
EarB (1)SNoneMK308836a-a-a-aMK308761-MK308786-MK308811-MK3087362K1N1i
EarC (1)SNoneMK308837a-a-a-aMK308762-MK308787-MK308812-MK3087372K1N1c
EarC (1)SNoneMK308838a-a-a-aMK308763-MK308788-MK308813-MK3087382K1N1d
EarC (1)RNoneMK308839a-a-a-aMK308764-MK308789-MK308814-MK3087392K1N1j
2008Ear/E1D (1)SNoneMK308840a-a-a-aMK308765-MK308790-MK308815-MK3087402K1N1c
Ear/E2D (1)RNoneMK308841a-a-a-aMK308766-MK308791-MK308816-MK3087412K1N1k
Ear/E3D (1)RNoneMK308842a-a-a-aMK308767-MK308792-MK308817-MK3087422K1N1a
2009Blood/B2E (1)SNoneMK294624a-a-a-aMK294579-MK294609-MK294594-MK2945642K1N1l
Blood/B3F (1)SNoneMK294625a-a-a-aMK294580-MK294610-MK294595-MK2945652K1N1c
2012EarD (1), G (2), H (1)SNoneB1-100%da-a-a-aB1-100%2NTfNT
EarD (1), G (1), I (1)RNoneB1-100%a-a-a-aB1-100%2NTNT
2014EarA (1)RK143RMK308843a-a-a-aMK308768-MK308793-MK308818-MK3087432NTNT
EarA (5)RNoneB1-100%a-a-a-aB1-100%2NTNT
2015EarA (1)RK143RMK308844a-a-a-aMK308769-MK308794-MK308819-MK3087442NTNT
EarA (1), J (1)SNoneB1-100%a-a-a-aB1-100%2NTNT
EarA (2)RNoneB1-100%a-a-a-aB1-100%2NTNT
2016EarA (1)RL43HMK308845a-a-a-aMK308770-MK308795-MK308820-MK3087452NTNT
EarA (2), J (1)SNoneB1-100%a-a-a-aB1-100%2NTNT
EarA (3)RNoneB1-100%a-a-a-aB1-100%2NTNT
2017Blood/B4K (1)RNoneMK294626a-a-a-aMK294581-MK294611-MK294596-MK2945662K1N1c
Ear/E9A (1)RNoneMK308847a-a-a-aMK308772-MK308797-MK308822-MK3087472K1N1c
EarJ (1)RK143RMK308848a-a-a-aMK308773-MK308798-MK308823-MK3087482NTNT
EarJ (1)RQ357KMK308849a-a-a-aMK308774-MK308799-MK308824-MK3087492K1N1c
EarA (1), J (1), L (1)SNoneB1-100%a-a-a-aB1-100%2NTNT
EarA (5), M (1)RNoneB1-100%a-a-a-aB1-100%2NTNT
2018EarA (1)SNoneMK308850a-a-a-aMK308775-MK308800-MK308825-MK3087502NTNT
EarA (2)RNoneB1-100%a-a-a-aB1-100%2NTNT
  • a Fluconazole resistance (FR) was categorized as resistant (R) or susceptible (S) according to the tentative fluconazole MIC breakpoints (32 µg/ml) for C. auris published by the U.S. Centers for Disease Control and Prevention (CDC) (https://www.cdc.gov/fungal/candida-auris/recommendations.html).

  • b The amino acid substitutions (AAS) in each isolate were analyzed based on the reference ERG11 sequence of C. auris (GenBank accession no. MK059959).

  • c Pulsed-field gel electrophoresis (PFGE) typing consisted of electrophoretic karyotyping (EK) and restriction endonuclease analyses of genomic DNA using NotI (REAG-N). Lower case letters (a to l) in REAG-N analysis indicate subgroups of a given (N1) type with banding patterns that are identical or differ by three or fewer bands.

  • d B1-100%, all isolates in the same row (n = 2 to 6 isolates) were identical at the DNA sequence level to isolate B1.

  • e MLST, multilocus sequence typing; ST, sequence type.

  • f NT, not tested.