TABLE 1

cfDNA isolation methodology of studiesa on blood- and urine-based cfDNA detection of M. tuberculosis by nucleic acid amplification techniques in which the methodological steps are a priori considered suitable for cfDNA isolation and detectionb,c

Publication’s first authorYrSample typeCentrifugation, urine supernate collectionPreservative/storageDNA extraction methodTest typeTarget(s)Amplicon target size(s) (bp)
Ushio2016PlasmaNAEDTA/NRQiagen DNeasy blood and tissue kitDigital PCRIS6110, gyrB71
Click2018PlasmaNAEDTA/NRQiaAmp circulating nucleic acid kitqPCRIS6110106
Cannas2008UrineYesEDTA/NRManual/resinNested PCRIS611067 and 129
Fortun2014UrineNRNR/NRNRTMA16S rRNANR
Labugger2017UrineYesEDTA/NRManual/resinPCRIS611038
Patel2017UrineNREDTA/NRManual/resinPCRDR region38
  • a See references 23, 24, 25, 32, 33, and 34.

  • b Two additional studies reported one case report (63, 64). Both studies describe the identification of urinary M. tuberculosis cfDNA in extrapulmonary TB cases; the first refers to a disseminated TB case while the second to a pediatric tubercular otitis media case. Sample preanalytical steps were performed as reported in the Ushio et al. study and Cannas et al. study, respectively (63, 64). Data from these studies were not included here given that only samples from an individual patient were available.

  • c NR, not reported; NA, not applicable; TMA, transcription-mediated amplification; DR, direct repeat.