TABLE 1

Characterization of reactions between MAb and NS1 proteins of DENV

HybridomaIsotypeType of epitopeReactivity of the four DENV serotypes by:Specificity
Western blottingaELISAb
DENV1DENV2DENV3DENV4DENV1DENV2DENV3DENV4
53-1.1IgG2a(κ)Linear++++++++NS1
12-4.1IgG1(κ)Conformational++NS1
33-7.1IgG1(κ)Conformational++NS1
43-1.3IgG2a(κ)Conformational++NS1
22-1.5IgG1(κ)Conformational++NS1
  • a The lysates of C6/36 cells infected with different dengue virus serotypes were treated using SDS-PAGE sample buffer and gel electrophoresis, before being transferred to a nitrocellulose membrane and blotted with each MAb.

  • b Different NS1 antigens were immunoaffinity purified from the cell culture supernatants of Vero cells infected with the different serotypes of DENV. Microwell plates were coated with specific NS1 antigens and reacted with each MAb.