TABLE 2

Limits of detection for rifampin heteroresistance among triplicatesa

Strain IDRifampin resistance-conferring mutationsValues by RDT quantified by Deeplex
LPA-Hain (%LOD)1Deeplex (%QP)1LPA-Nipro (%LOD)1Deeplex (%QP)1Xpert (%LOD)1Deeplex (%QP)1Ultra (%LOD)2Deeplex (%QP)2
TB-TDR-0036H445DbH526Dc5–107.557.540–6055.940–60
TB-TDR-0090S450LbS531Lc551–5520–4021.720–3021.7
TB-TDR-0100D435VbD516Vc5–1065–10670–8069.640–50
TB-TDR-0131H445YbH526Yc5–104.3108.770–8066.760–70
  • a With quantification of the respective lowest proportion by targeted deep sequencing (Deeplex-MycTB). ID, identifier; Deeplex-MycTB, targeted deep sequencing; RDT, rapid diagnostic test; LPA-Hain, GenoTypeMTDBRplusv2.0; LPA-Nipro, GenoscholarNTM+MTBII; Xpert, classic XpertMTB/RIF; Ultra, Xpert MTB/RIF Ultra; 1, batch 1; 2, batch 2; %LOD, limit of detection (range) among triplicates for the RDTs; %QP, quantified proportion of minority resistant variants determined by Deeplex on replicate 3.

  • b In M. tuberculosis numbering.

  • c In E. coli numbering.