Table 4.

Limits of detection of the TLDA and IRTP assays

Assay and targetaLOD rangeUnit of measurebFold decrease in TLDA LOD
IRTP assayTLDA
Flu A
    Template A0.6–66–60EID50/ml (H1)10
    Template B6–6060–600EID50/ml (H3)10
Flu A–H10.6–66–60EID50/ml10
Flu A–H36–6060–600EID50/ml10
Flu B0.6–66–60EID50/ml10
RSV0.6–660–600Transcripts/μl100
HPIV160–600600–6000Transcripts/μl10
HPIV26–606–60Transcripts/μl1
HPIV36–6060–600Transcripts/μl10
HMPV600–6000600–6000Transcripts/μl1
RV0.6–66–60Transcripts/μl10
EV6–6060–600Transcripts/μl10
HPeV0.6–66–60Transcripts/μl10
Pan-AdV
    AV110.6–66–60GE/μl (AV11)10
    AV156–60600–6000GE/μl (AV15)100
Pan-Legionella spp.0.06–0.60.6–6GE/μl10
L. pneu0.06–0.60.6–6GE/μl10
H. influ0.6–60.6–6GE/μl1
S. pneumo0.6–66–60GE/μl10
S. pyo6–6060–600GE/μl10
M. pneumo0.06–0.60.6–6GE/μl10
C. pneumo0.04–0.44–40IFU/ml100
B. pert I6–606–60GE/μl1
B. pert II6–606–60GE/μl1
  • a Flu A, influenza virus type A; Flu A–H1, influenza virus type A subtype H1; RSV, respiratory syncytial virus; HPIV1, human parainfluenza virus type 1; HMPV, human metapneumovirus; RV, rhinovirus; EV, enterovirus; HPeV, human parechovirus; pan-AdV, pan-adenovirus; AV11, adenovirus 11; L. pneu, Legionella pneumophila; H. influ, Haemophilus influenzae; S. pneumo, Streptococcus pneumoniae; S. pyo, Streptococcus pyogenes; M. pneumo, Mycoplasma pneumoniae; C. pneumo, Chlamydophila pneumoniae; B. pert I, Bordetella pertussis target I.

  • b EID50, 50% egg infectious dose; GE, genome equivalents; IFU, inclusion-forming units.