Table 5.

Sensitivity and specificity of TLDA assays compared to individual real-time PCR assays using a CT cutoff of 43

TargetaNo. of resultsSensitivity (%) (95% CIb)Specificity (%) (95% CI)
True positiveFalse positiveFalse negativeTrue negativeTotal
Flu A22127710292 (80–100)99 (96–100)
Flu A–H160094100100 (100–100)100 (100–100)
Flu A–H315138310283 (65–100)99 (96–100)
Flu B14007286100 (100–100)100 (100–100)
RSV302075107100 (100–100)97 (94–100)
HPIV17007481100 (100–100)100 (100–100)
HPIV21123749079 (55–100)96 (92–100)
HPIV32101739595 (86–100)100 (100–100)
HMPV1911749595 (85–100)99 (96–100)
RV41616911798 (93–100)91 (84–97)
EV13607392100 (100–100)92 (86–98)
HPeV1007980100 (100–100)100 (100–100)
Pan-AdV28018111097 (90–100)100 (100–100)
Pan-Legionella spp.19167610277 (60–94)99 (96–100)
L. pneu19167610277 (60–94)99 (96–100)
H. influ15307593100 (100–100)96 (92–100)
S. pneumo13510115521192 (88–97)85 (76–94)
S. pyo3007881100 (100–100)100 (100–100)
M. pneumo8098410150 (25–75)100 (100–100)
C. pneumo16087810267 (47–87)100 (100–100)
B. pert I101093104100 (100–100)99 (97–100)
B. pert II703748470 (38–100)100 (100–100)
Total46035561,6872,23889 (86–92)98 (97–99)
  • a Flu A, influenza virus type A; Flu A–H1, influenza virus type A subtype H1; RSV, respiratory syncytial virus; HPIV1, human parainfluenza virus type 1; HMPV, human metapneumovirus; RV, rhinovirus; EV, enterovirus; HPeV, human parechovirus; pan-AdV, pan-adenovirus; L. pneu, Legionella pneumophila; H. influ, Haemophilus influenzae; S. pneumo, Streptococcus pneumoniae; S. pyo, Streptococcus pyogenes; M. pneumo, Mycoplasma pneumoniae; C. pneumo, Chlamydophila pneumoniae; B. pert I, Bordetella pertussis target I.

  • b CI, confidence interval.