Table 3.

Characteristics of E. coli J53 transconjugants carrying blaCTX-M genes described in this studya

TransconjugantDonor strainbla gene transferredApproximate plasmid size (kb)Replicon type by PCRISEcp1 upstream of blaCTX-MIS903 downstream of blaCTX-MResistance transferred
p9V0615-1J09-V06-15CTX-M-1595FIIs+AMP CEP XNL CTX ATM GEN STR TET SUL
p9V0616-1J09-V06-16CTX-M-1595FIIs+AMP CEP XNL CTX ATM GEN STR TET SUL
p9V0620-1J09-V06-20CTX-M-1595FIIs+AMP CEP XNL CTX ATM GEN STR TET SUL
p9V0621-1J09-V06-21CTX-M-1595FIIs+AMP CEP XNL CTX ATM GEN STR TET SUL
p9V0622-1J09-V06-22CTX-M-1595FIIs+AMP CEP XNL CTX ATM GEN STR TET SUL
p9V0623-1J09-V06-23CTX-M-1595FIIs+AMP CEP XNL CTX ATM GEN STR TET SUL
p9V0628-1J09-V06-28CTX-M-1595FIIs+AMP CEP XNL CTX ATM GEN STR TET SUL
p9V0558-1J09-V05-58CTX-M-1595FIIs+AMP CEP XNL CTX ATM GEN STR TET SUL
p9G004-1J09-G-004CTX-M-1595FIIs+AMP CEP XNL CTX ATM GEN STR TET SUL
p9G097-1J09-G-097CTX-M-1595FIIs+AMP CEP XNL CTX ATM GEN STR TET SUL
p9G063-1J09-G-063CTX-M-1595FIIs+AMP CEP XNL CTX ATM GEN STR TET SUL
p9G069-1J09-G-069CTX-M-1495I1-Iγ++AMP CEP XNL CTX
p9G076-1J09-G-076CTX-M-1495I1-Iγ++AMP CEP XNL CTX
  • a Abbreviations: AMP, ampicillin; CEP, cephalothin; XNL, ceftiofur; CTX, cefotaxime; ATM, aztreonam; GEN, gentamicin; STR, streptomycin; TET, tetracycline; SUL, sulfamethoxazole; ISEcp1, insertion sequence ISEcp1; IS903, insertion sequence IS903; +, positive; −, negative.