Table 1.

Sample spiking schemea

VirusbTitration methodGenome structureSample identification (titer)
PlasmaCSFBloodCell supernatant
P10P1000P100000CSF10CSF100CSF1000B100B1000B10000S3LODxS0.8LODx
RABVFFU or ccssRNA10103.0105.010102103102103104
PVPFU or cc10103.0105.010102103102103104
HHV-1PFU or ccdsDNA10103.0105.0102103104
HHV-4gc103.8104.3104.7
RhCMVTCID5028
HADV-5TCID50103.0102.4
HBVIU104.0103.4
B19IUssDNA103.0102.4
BVDV-1-NADLTCID50ssRNA216
H3N8gc103.0102.4
FLUBV-B/LEE/40gc103.0102.4
HCVIU103.5102.9
  • a The titers of viruses spiked in plasma, CSF, or in MRC5 cell supernatants are expressed per ml (FFU, focus-forming unit; gc, genome copies; IU, international units roughly equivalent to gc; TCID50: 50% tissue culture infectious dose). Virus-infected cells were spiked in blood, and these concentrations are given as the number of virus-infected cells per ml of blood (cc). LOD, limit of detection of quantitative (RT-)PCR tests.

  • b Abbreviations: BVDV-1-NADL, bovine viral diarrhea virus; B19, parvovirus B19 (B19) (NIBSC 99/800); FLUBV-B/LEE/40, influenza B virus; HADV-5, adenovirus type 5; H3N8, equine influenza virus A/Equine/2/Miami/1/63; HBV, hepatitis B virus; HCV, hepatitis C virus; HHV-1, human herpesvirus type 1; PV, poliovirus Sabin; RABV, rabies virus; RhCMV, rhesus cytomegalovirus.