Table 2.

Algorithm of the multitarget real-time PCR assay

SpeciesAssay result
Singleplex ptxS1Multiplex
IS481hIS1001pIS1001
B. pertussis++
B. parapertussisa++
B. holmesii++
B. pertussis and B. parapertussisa+++
B. pertussis and B. holmesiib+++
  • a A specimen positive for pIS1001 may be considered to most probably contain B. parapertussis, but the possibility that it is positive for B. bronchiseptica cannot be totally excluded.

  • b This coinfection has been documented by culture in our laboratory.