Table 1.

Overview of bacteria found in the explanted lung samples by culturing and 16S rRNA gene analysis

PatientCulturing16S rRNA gene analysis
Species (BLAST)aOTUNo.b
Patient 1Achromobacter xylosoxidansAchromobacter xylosoxidans1115
Uncultured bacterium2*c3
Burkholderia fungorum32
Uncultured bacterium4*2
Bacillus cereus51
Uncultured Flavobacterium6*1
Uncultured bacterium7*1
Uncultured bacterium8*1
Uncultured bacterium9*1
Uncultured bacterium10*1
Patient 2Pseudomonas aeruginosaPseudomonas aeruginosa1118
Lactobacillus mucosae21
Patient 3Pseudomonas aeruginosaPseudomonas aeruginosa1406
Uncultured Bacteroidetes bacterium227
Stenotrophomonas maltophilia311
Uncultured bacterium4*3
Uncultured bacterium5*2
Uncultured Saprospiraceae bacterium61
Uncultured Bacteroidetes bacterium71
Uncultured bacterium8*1
Uncultured bacterium9*1
Uncultured bacterium10*1
Patient 4Pseudomonas aeruginosaPseudomonas aeruginosa1191
Stenotrophomonas maltophiliaStenotrophomonas maltophilia26
Uncultured bacterium32
Uncultured betaproteobacterium41
Uncultured betaproteobacterium5*1
Uncultured betaproteobacterium61
Polaromonas sp.71
Uncultured bacterium8*1
Uncultured bacterium91
Patient 5Achromobacter xylosoxidansAchromobacter xylosoxidans1440
Stenotrophomonas maltophilia21
  • a The species found by 16S rRNA gene analysis is given by the closest relatives of the bacterial OTUs in clone libraries for the patients.

  • b The number of sequences that make up the OTUs.

  • c Asterisks indicate clones where the identification by BLAST differed from the identification made by phylogenetic analysis.