Table 4

Performance of FilmArray RP for detection of characterized adenovirus serotypes at high and low viral loadsa

Adenovirus type (ATCC no.)High viral loadLow viral load
PCR CT valuePCR DNA copies/ml (log10)FilmArray RP resultPCR CT valuePCR DNA copies/ml (log10)FilmArray RP result
Group A
    ATCC HAdv-12 (VR-863)26.053.41 × 107 (7.53)Positive36.242.12 × 104 (4.33)Positive
Group B
    LRRI 3p26.161.75 × 107 (7.24)Positive37.431.46 × 104 (4.16)Positive
    ATCC HAdv-7 (VR-7)25.704.24 × 107 (7.63)Positive35.349.89 × 104 (5.00)Positive
    ATCC HAdv-11 (VR-12)26.472.61 × 107 (7.42)Positive35.902.63 × 104 (4.42)Positive
    LRRI 14p27.201.65 × 107 (7.22)Positive36.513.62 × 104 (4.56)Positive
    ATCC HAdv-21 (VR-256)23.775.47 × 107 (7.74)Positive37.243.03 × 104 (4.48)Positive
    ATCC HAdv-35 (VR-718)28.306.02 × 106 (6.78)Positive37.978.25 × 103 (3.92)Negative
Group C
    ATCC HAdv-1 (VR-1)27.875.98 × 106 (6.78)Positive35.917.03 × 104 (4.85)Positive
    ATCC HAdv-2 (VR-846)25.881.51 × 107 (7.18)Positive37.459.35 × 103 (3.97)Negative
    LRRI 227.169.29 × 106 (6.97)Positive37.241.64 × 104 (4.22)Negative
    LRRI 5p27.921.05 × 107 (7.02)Positive35.151.12 × 105 (5.05)Positive
    ATCC HAdv-6 (VR-6)26.842.07 × 107 (7.32)Negative
Group D
    LRRI 1027.189.16 × 106 (6.96)Positive36.503.64 × 104 (4.56)Positive
    ATCC HAdv-19 (VR-254)25.242.18 × 107 (7.34)Positive34.914.97 × 104 (4.70)Positive
    LRRI 20p26.371.06 × 107 (7.03)Positive37.142.45 × 104 (4.39)Negative
    ATCC HAdv-29 (VR-272)26.778.27 × 106 (6.92)Positive36.271.04 × 104 (4.02)Positive
    ATCC HAdv-32 (VR-625)27.707.13 × 106 (6.85)Positive36.301.04 × 104 (4.02)Positive
    ATCC HAdv-37 (VR-929)26.411.57 × 107 (7.20)Positive36.701.60 × 104 (4.20)Negative
Group E
    ATCC HAdv-4 (VR-4)24.463.54 × 107 (7.55)Positive35.034.55 × 104 (4.66)Positive
Group F
    ATCC HAdv-40 (VR-931)26.891.17 × 107 (7.07)Positive36.613.39 × 104 (4.53)Positive
    ATCC HAdv-41 (VR-930)27.209.61 × 106 (6.98)Negative
  • a Abbreviations: ATCC, American Type Culture Collection; LRRI, Lovelace Respiratory Research Institute. Real-time PCR CT values and viral loads represent means of multiple replicates. Repeat testing was performed on all discordant results.