Table 3

Lower limits of detection of the real-time PCR assays

AssayOrganismGenome DNA equivalents/PCR
MultiplexaNew singleplexPrevious singleplex
N. meningitidis ctrAN. meningitidis B1823NAb
N. meningitidis Y1412NA
H. influenzae hpdHib4246NA
HiNTc2323NA
S. pneumoniae lytAS. pneumoniae type 411NA
S. pneumoniae type 6B11NA
Serogroup B synDN. meningitidis B9966,171
Serogroup C synEN. meningitidis C2917NA
Serogroup Y synFN. meningitidis Y11108,955
Serogroup A sacBN. meningitidis A210221NA
Serogroup W135 synGN. meningitidis W13548431,088,161
Serogroup X xcbBN. meningitidis X1120NA
  • a The multiplex assays were the NHS assay, detecting N. meningitidis, H. influenzae, and S. pneumoniae; the BCY assay, detecting N. meningitidis serogroups B, C, and Y; and the AWX assay, detecting N. meningitidis serogroups A, W135, and X.

  • b NA, not applicable.

  • c HiNT, nontypeable H. influenzae.