Table 4

Sensitivities and specificities of the multiplex and singleplex real-time PCR assays for detection of meningitis pathogens from clinical specimens

Specimen group (reference standard)Assay (type)aNo. positive/no. testedSensitivity (95% CI)No. negative/no. testedSpecificity (95% CI)
Lackland (fucK PCR)H. influenzae hpd (M)206/24484.4 (79.3–88.7)217/24389.3 (84.7–92.9)
H. influenzae hpd (S)206/24484.4 (79.3–88.7)218/24389.7 (85.2–93.2)
Brazil (CIEb)N. meningitidis ctrA (M)72/7596.0 (88.8–99.2)8/2433.3 (15.6–55.3)
N. meningitidis ctrA (S)72/7596.0 (88.8–99.2)9/2437.5 (18.8–59.4)
Serogroup C synE (M)71/7594.7 (86.9–98.5)17/2470.8 (48.9–87.4)
Serogroup C synE (S)72/7596.0 (88.8–99.2)17/2470.8 (48.9–87.4)
South Africa (compositec)N. meningitidis ctrA (M)56/7673.7 (62.3–83.1)43/4497.7 (88.0–99.9)
N. meningitidis ctrA (S)58/7676.3 (65.2–85.3)37/4484.1 (69.9–93.4)
H. influenzae hpd (M)16/2176.2 (52.8–91.8)98/9999.0 (94.5–100.0)
H. influenzae hpd (S)18/2185.7 (63.7–97.0)91/9991.9 (84.7–96.4)
S. pneumoniae lytA (M)16/1794.1 (71.3–99.8)102/10399.0 (94.7–100.0)
S. pneumoniae lytA (S)16/1794.1 (71.3–99.8)96/10393.2 (86.5–97.2)
  • a M, multiplex; S, singleplex.

  • b One specimen was not tested by CIE and was therefore excluded from this analysis.

  • c Combined laboratory tests (culture and/or latex agglutination) were used as the composite reference standard, where a positive result was defined as culture or latex agglutination positive (negative otherwise). One specimen was not tested by culture or latex agglutination and was therefore excluded from this analysis.