Table 2

Analytical specificities of pan-Rickettsia and R. rickettsii real-time assays

PanelNo. of samples testedResult
PanR8RRi6
Rickettsia species (excluding R. rickettsii)a13PositiveNegative
Rickettsia rickettsii isolates31PositivePositive
Rickettsia prowazekii isolates34PositiveNegative
Rickettsia typhi isolates20PositiveNegative
Rickettsia near neighborsb15NegativeNegative
Bacteria with similar clinical symptomsc11NegativeNegative
Human DNA, tissuesd8NegativeNegative
Protozoan speciese4NegativeNegative
Fungal speciesf5NegativeNegative
Bacterial speciesg27NegativeNegative
  • a Rickettsia akari, R. amblyommii, R. australis, R. canadensis, R. conorii, R. felis, R. honei, R. massiliae, R. montana, R. parkeri, R. rhipicephali, R. sibirica, and R. slovaca.

  • b Bartonella henselae, Bartonella quintana, Bartonella vinsonii subsp. berkhoffii, Coxiella burnetii, Ehrlichia chaffeensis (7 strains), Ehrlichia muris, and Orientia tsutsugamushi (3 strains).

  • c Borrelia burgdorferi, Brucella ovis, Francisella tularensis, Leptospira interrogans, Listeria monocytogenes, Legionella pneumophila, Neisseria meningitides, Neisseria gonorrhoeae, and Salmonella enterica (3 strains).

  • d Blood, heart, kidney, liver, lung, peripheral blood leukocyte, skin, spleen.

  • e Acanthamoeba castellanii, Cryptosporidium parvum, Giardia intestinalis, Toxoplasma gondii.

  • f Aspergillus oryzae, Candida albicans, Penicillium marneffei, Saccharomyces cerevisiae, Trichoderma reesei.

  • g Enterococcus faecalis, Escherichia coli (2 strains), Helicobacter pylori, Klebsiella pneumoniae, Mycoplasma pneumoniae, Peptostreptococcus anaerobius, Propionibacterium acnes, Pseudomonas aeruginosa (3 strains), Pseudomonas putida, Shigella flexneri, Serratia marcescens, Staphylococcus aureus (3 strains), Staphylococcus epidermidis (4 strains), Streptococcus gallolyticus, Streptococcus pneumoniae, Streptococcus pyogenes (2 strains), Vibrio cholerae, and Yersinia pestis.