Table 1

Multiplex real-time analysis of Legionella-positive and -negative clinical specimens

Sample no.SourcecCulture resultDFAMultiplex real-time PCR CT value
Legionella spp. (ssrA)L. pneumophila (mip)L. pneumophila sg1 (wzm)Human DNA (RNase P)
1Sputum+sg127.6326.8427.7425.96
2Lung+sg117.1516.8517.1117.66
3Lung+sg115.1414.9715.1916.56
4Br. swab+sg125.5324.4724.5323.91
5Lung+sg126.8926.1825.9520.00
6Lung+sg128.5528.0726.9817.66
7Lung+sg125.1924.3724.1719.59
8Lung+sg125.9325.3024.7219.05
9Lung+sg129.3628.7428.2720.50
10Lung+sg126.0425.1825.1517.50
11Lung+sg126.5425.9725.8121.24
12Lung+sg126.7227.0726.5219.83
13BAL+sg133.1732.1331.9724.70
14Sputum+sg127.8827.2126.6616.75
15Lung+sg128.1827.2426.9520.48
16OP swabNTbNDcNDND24.11
17UrineNTNDNDND23.07
18OP swabNTNDNDND25.17
19NP swabNTNDNDND24.56
20SputumNTNDNDND22.26
21SputumNTNDNDND23.98
  • a Br. swab, OP swab, and NP swab, bronchial, oropharyngeal, and nasopharyngeal swabs, respectively; BAL, bronchoalveolar lavage specimen.

  • b NT, not tested.

  • c ND, not detected.