Table 2

Primers for PCR and sequencing of IMP genes

Target(s)Primer nameUsePrimer sequence (5′ to 3′)Product length (bp)
IMP-1, -3, -6, -10, -25, -30, -40, -42IMP-1 whole-M13FAmplificationTGTAAAACGACGGCCAGTATGAGCAAGTTATCTGTATTC777
IMP-1 whole-M13RCAGGAAACAGCTATGACCTTAGTTGCTTGGTTTTGATGG
IMP-7IMP-7 whole-M13FAmplificationTGTAAAACGACGGCCAGTATGAAAAAGTTATCAGTATTC777
IMP-7 whole-M13RCAGGAAACAGCTATGACCTTAGTTACTTGGTTTTGATAG
IMP-2, -8, -24IMP-2 whole-M13FAmplificationTGTAAAACGACGGCCAGTATGAAGAAATTATTTGTTTTATG777
IMP-2 whole-M13RCAGGAAACAGCTATGACCTTAGTTACTTGGCTGTGATGG
IMP-11, -21, -41IMP-11 whole-M13FAmplificationTGTAAAACGACGGCCAGTATGAAAAAACTATTTGTTTTATGT774
IMP-11 whole-M13RCAGGAAACAGCTATGACCTTAATGAACAGTGTTACTTT
IMP-19, -20IMP-19 whole-M13FAmplificationTGTAAAACGACGGCCAGTATGAAGAAACTATTTGTTTTATGT777
IMP-2 whole-M13RCAGGAAACAGCTATGACCTTAGTTACTTGGCTGTGATGG
Universal sequenceM13FSequenceTGTAAAACGACGGCCAGT
M13RSequenceCAGGAAACAGCTATGACC